When plugged into a device, the USB Killer rapidly charges its capacitors from the USB power lines. When the device is charged, -200VDC is discharged over the data lines of the host device. This charge/discharge cycle is repeated many times per second, until the USB Killer is removed. Simply put: used on unprotected equipment, the USB Killer instantly and permanently disables unprotected hardware.
Source: USB Killer – ESD Tester to test and disable USB devices
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